娘汾体狄佛芒灸究侗特羞丛袜
Biomembrane Structure
Biomembrane Structure-test
1、单选题：
All the following statements describe biomembranes except:
A: Different biomembranes may contain different proportions of the same phospholipids.
B: The two leaflets of a biomembrane may contain different phospholipids.
C: Some biomembranes have free edges.
D: Some phospholipids and cholesterol may cluster to form lipid rafts.
答案: Some biomembranes have free edges.
2、单选题：
The major site of lipid synthesis in eukaryotic cells is the
A: Nucleus
B: Endoplasmic reticulum
C: Mitochondria
D: Cell membrane
答案: Endoplasmic reticulum
3、单选题：
Phospholipids with short or unsaturated fatty acyl chains
A: decrease membrane fluidity.
B: increase membrane fluidity.
C: cause biomembranes to become thicker
D: allow hydrophilic molecules to diffuse across the lipid bilayer.
答案: increase membrane fluidity.
4、单选题：
The _______ are transmembrane proteins.
A: lipid-anchored membrane proteins
B: integral membrane proteins
C: peripheral membrane proteins
D: extracellular matrix proteins
答案: integral membrane proteins
5、单选题：
Glycolipids and glycoproteins are especially abundant in the
A: nucleus.
B: mitochondrial inner membrane
C: cytosol
D: plasma membrane
答案: plasma membrane
6、单选题：
Phosphate containing lipids include all of the following except
A: phospholipids.
B: sphingolipid
C: triglyceride
D: phosphatidylinositol
答案: triglyceride
7、判断题：
A lipid bilayer is the fundamental structural component of all cell membranes.
A: 正确
B: 错误
答案: 正确
8、判断题：
Maintenance of the lipid bilayer in the plasma membrane requires special enzymes and the hydrolysis of ATP.
A: 正确
B: 错误
答案: 错误
9、填空题：
The most abundant lipids in cell membranes are ________________.
答案: phospholipids
10、填空题：
Sugar-containing lipids called ____________ are found only in the outer half of the bilayer and their sugar groups are exposed ac the cell surface.
答案: glycolipids
Cell organization and movement I Microfilaments
Cell organization and movement I
Cell organization and movement II Microtubules and intermediate filament
Cell organization and movement II
Cell senescence and death
Cell senescence and death
1、单选题：
Which caspase is involved in NON-canonical inflammasome signaling?
A: caspase-9
B: caspase-3
C: caspase-1
D: caspase-11
答案: caspase-11
2、单选题：
Which protein is the executor of necroptosis?
A: RIP3
B: MLKL
C: GSDMD
D: caspase-7
答案: MLKL
3、单选题：
Which one is NOT the morphological characteristics of apoptotic cells?
A: cell shrinkage
B: DNA fragmentation
C: plasma membrane rupture
D: blebbing
答案: plasma membrane rupture
4、单选题：
Which one is involved in senescent phenotype exacerbation, consisting of inflammatory cytokines, MMP, growth factors etc.?
A: SASP
B: SAHF
C: DAMPs
D: PAMPs
答案: SASP
5、单选题：
Which one is the molecular switch from apoptosis to necroptosis?
A: RIP1
B: RIP3
C: caspase-8
D: TNFα
答案: RIP3
6、多选题：
Which one is the physiological roles of apoptosis during normal development?
A: to prevent webbed hands and embryonic tails
B: to prevent the existence of useless nerve connections
C: to induce inflammatory responses against foreign pathogens
D: to function in immune cell selection
答案: to prevent webbed hands and embryonic tails;
to prevent the existence of useless nerve connections;
to function in immune cell selection
7、多选题：
Which of the following is NOT true?
A: At the late stage of apoptosis, plasma membrane is intact, and the cell corpses are ingested by neighboring cells
B: Quiescent cells can re-enter the cell cycle while senescent cells can not because senescent cells are metabolically inactive
C: Apoptotic cells can be stained positively when subjected to acidic lysosomal SA-β-GAL staining
D: Caspase cascade is dominant in apoptotic signaling while phosphorylation cascade plays important roles in necroptotic signal transduction
答案: Quiescent cells can re-enter the cell cycle while senescent cells can not because senescent cells are metabolically inactive;
Apoptotic cells can be stained positively when subjected to acidic lysosomal SA-β-GAL staining
8、多选题：
Which of the following is NOT morphology of senescent cells?
A: increased cell size
B: ruptured plasma membrane
C: blebbing and formation of bubbles
D: flat and enlarged cytoplasm
答案: ruptured plasma membrane;
blebbing and formation of bubbles
9、多选题：
The activation of which caspase could NOT be considered as marker of apoptosis?
A: caspase-1
B: caspase-3
C: caspase-7
D: caspase-11
答案: caspase-1;
caspase-11
10、判断题：
Senescent cells only exist in the old.
A: 正确
B: 错误
答案: 错误
11、判断题：
Cell death will no doubt lead to the death of the organism.
A: 正确
B: 错误
答案: 错误
Cellular Energetics
Cellular Energetics
1、单选题：
Where does glycolysis takes place?
A: Mitochondrial matrix
B: Cytosol
C: Mitochondrial intermembrane space
D: Mitochondrial inner membrane
E: Mitochondrial outer membrane
答案: Cytosol
2、单选题：
Where is the electron transport chain found in plant cells?
A: Stroma of chloroplasts
B: Inner membrane of mitochondria
C: Thylakoid membranes of chloroplasts
D: Cytoplasm
答案: Thylakoid membranes of chloroplasts
3、单选题：
Energy released by the electron transport chain is used to pump H+ ions into which location?
A: Mitochondrial matrix
B: Mitochondrial intermembrane space
C: Mitochondrial inner membrane
D: Cytosol
答案: Mitochondrial intermembrane space
4、单选题：
Inside an active mitochondrion, most electrons follow which pathway?
A: Glycolysis –> NADH –> oxidative phosphorylation –> ATP –> oxygen
B: Citric acid cycle –> FADH2 –> electron transport chain –> ATP
C: Pyruvate –> citric acid cycle –> ATP –> NADH –> oxygen
D: Citric acid cycle –> NADH –> electron transport chain –> oxygen
答案: Citric acid cycle –> NADH –> electron transport chain –> oxygen
5、单选题：
Which do the catabolic products of fatty acid breakdown enter into the citric acid cycle?
A: Pyruvate
B: Acetyl CoA
C: Succinyl CoA
D: Malate or fumarate
答案: Acetyl CoA
6、单选题：
Brown-fat mitochondria uncouple oxidative phosphorylation to produce__________
A: ADP
B: Fat
C: Heat
D: Oxygen
答案: Heat
7、判断题：
During ATP synthesis, protons move “down” their electrochemical gradient through the F0 complex of ATP synthase
A: 正确
B: 错误
答案: 正确
8、判断题：
The outer mitochondrial membrane is derived from the bacterial plasma membrane
A: 正确
B: 错误
答案: 错误
9、填空题：
__________ is a lipid soluble molecule that acts to shuttle electrons within the mitochondrial inner membrane
答案: CoQ
10、填空题：
The energy released by the passage of electrons along the respiratory chain is stored as an___________ across the inner mitochondrial membrane.
答案: proton motive force
Culturing and visualizing cells
Culturing and visualizing cells-test
1、单选题：
The most direct source of monoclonal antibodies are ________ cells.
A: B lymphocytes
B: T lymphocytes
C: myeloma
D: Hybridoma
答案: Hybridoma
2、单选题：
The fluorescent properties of dyes such as FITC can provide information on the
A: location of specific proteins.
B: concentration of ions in specific regions of the cell.
C: the amount of DNA in a cell.
D: volume of a cell.
答案: location of specific proteins.
3、单选题：
What factors necessary for growth of animal cells in culture are provided by serum?
A: amino acids
B: precursors of DNA synthesis
C: growth factors
D: vitamins
答案: growth factors
4、单选题：
What is the advantage of using GFP-tag to visualize specific proteins, instead of staining cells with fluorescent dyes that bind to the target protein?
A: GFP fusions enable one to track changes in the location of the protein in living cells; staining usually requires fixed cells
B: GFP fusions enable higher resolution than staining with fluorescent probes.
C: GFP permits the position of the protein in the cell more precisely than fluorescent probes
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